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. 2012 Mar 2;127(1):120–129. doi: 10.1093/toxsci/kfs099

FIG. 2.

FIG. 2.

Effect of arsenite on UV-induced cleavages of PARP-1, caspase-3, -7, and -9 and release of Cyto C. HaCat cells were incubated with 2μM arsenite for 24 h before UV exposure. Twelve hours after UV radiation, nuclear and cytoplasmic cell extracts were prepared. Protein levels of cleaved caspase-3, -7, and -9 were determined by Western blot (A). Six hours after UV radiation, mitochondrial and cytoplasmic extracts were prepared. The release of Cyto C from mitochondria to the cytoplasm was detected by Western blot (B). The band intensities were quantified by KODAK Molecular Imaging Software version 4.0. Data represent the mean ± SD of three independent experiments. *p < 0.05 versus control and §p < 0.05 versus UV.