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Effect of PARP-1 knockdown on UV-induced apoptosis. HaCat cells were transfected with control or PARP-1 siRNA. Seventy-two hours after transfection, cells were incubated with 2μM arsenite for 24 h before UV exposure. Twelve hours after UV radiation, cell apoptosis was measured by flow cytometry using the TACS Annexin V kit (A). Percentages of cell apoptosis, necrosis, and viability were calculated (B). §p < 0.05 versus UV.
