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. 2012 Mar 2;127(1):120–129. doi: 10.1093/toxsci/kfs099

FIG. 5.

FIG. 5.

Effect of PARP-1 knockdown on UV-induced PARP-1, caspase cleavages, releases of Cyto C, and AIF and PAR formation. HaCat cells were transfected with control or PARP-1 siRNA. Seventy-two hours after transfection, cells were incubated with 2μM arsenite for 24 h before UV exposure. Cells were collected 6 h after UV radiation for assessment of PAR production and Cyto C and AIF release in cytosolic fraction. Cells were collected 12 h after UV radiation for detection of PARP-1 and caspase cleavages in nuclear extract by Western blot (A). The band intensities were quantified by KODAK Molecular Imaging Software version 4.0 (B and C). Data represent the mean ± SD of three independent experiments. §p < 0.05 versus UV.