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Diagram of a fluorescence-based assay, like the Fluor de Lys (BIOMOL), displaying reactants and products of the reaction used to monitor Sirtuin activity.[2a, 36a] The p53-derived acetyl lysine peptide is covalently conjugated to a 7-amino-4-methylcoumarin (AMC) fluorophore. Deacetylation sensitizes the lysine residue to a trypsin developer that cleaves at the C-terminal end of deacetylated lysine and exposes the fluorescent tag resulting in an increase in fluorescent signal.
