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. Author manuscript; available in PMC: 2012 Aug 14.
Published in final edited form as: Dev Cell. 2012 Feb 14;22(2):309–319. doi: 10.1016/j.devcel.2011.12.025

Figure 7. Phosphorylation of β-catenin on tyrosine 667 regulates β-catenin dynamics and rosette formation.

Figure 7

(A,B) The percentage of pre-bleach fluorescence after bleaching AP junctions in embryos expressing Venus-tagged β-catΔC, β-catΔC667E, or β-catΔC667F. (C) β-catΔC667E had increased mobility compared to β-catΔC (p=0.0009) and β-catΔC667F (p=0.0034). There was no difference in the mobility of β-catΔC and β-catΔC667F (p=0.5132) (n=21 β-catΔC, 18 β-catΔC667E, 23 β-catΔC667F). (D) The t½ was similar for all transgenes. (E, F) Stills from movies of β-catenin KD embryos expressing HA:β-catΔC667E or HA:β-catΔC667F. Cells labeled with Spider:GFP. A multicellular cable (black line) contracts to form a rosette in an embryo expressing β-catΔC667E (E) (new contacts, yellow line). Little contraction occurs in an embryo expressing β-catΔC667F (F). (G) Percentage of linked AP edges that joined a rosette of 5 or more cells in WT, abl, and β-cat KD embryos expressing the indicated β-catΔC transgenes. Fewer linked AP edges formed rosettes in abl (p=0.0421 vs. WT) and β-catΔC667F (p=0.0233 vs. β-catΔC and p=0.0149 vs. β-catΔC667E) (38–168 edges scored in 3–8 embryos/genotype). Error bars indicate the s.e.m. between embryos. (H) Percentage of fully extended embryos. 97% of Gal4 control embryos completed elongation (n=147 embryos) vs. 37% of abl KD embryos (n=527) (p<0.0001). 60% of abl KD embryos expressing full-length HA:β-catenin667E completed elongation (n=469) (p<0.0001 compared to abl KD), vs. 35% of abl KD embryos expressing full-length HA:β-catenin (n=239) (p=0.57) and 33% of abl KD embryos expressing full-length HA:β-catenin667F (n=511) (p=0.17). Bar, 5 µm. See also Figures S4,5.