Figure 5.
Expression of ABCC1, ABCG2 and MVP is independent of gain-of-function β-catenin. ABCC1, ABCG2 and MVP were not differentially expressed in cells with different β-catenin genotype. (A) ABCC1, ABCG2 and MVP mRNA expressions were determined by quantitative real-time RT–PCR; values represent the ratios of gene and G6PDH control mRNA (HCT116 cells: set 100%). Experiments were performed in duplicate and average values are shown. At the protein level, no significantly different expression levels of ABCC1, ABCG2 and MVP have been detected by western blot analysis (B) or by immuno flow cytometry (C, D).