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. 2012 Apr 16;443(Pt 3):821–828. doi: 10.1042/BJ20111491

Figure 2. saRNA-mediated NANOG overexpression in human embryonic carcinoma NCCIT cells.

Figure 2

(A) NCCIT cells were transfected with 50 nM concentrations of the indicated saRNAs for 96 h. Mock samples were transfected in the absence of saRNA. NANOG expression was assessed by quantitative RT–PCR. Results are the fold changes relative to mock transfections (means±S.D. for three independent experiments). *P< 0.05 compared with the mock. (B) NCCIT cells were transfected with mock, dsControl, dsNanog-752 or dsNanog-926 for 72 h. NANOG protein level was detected by immunoblot analysis. Hsp70 served as a loading control. (C) Representative phase contrast cell images were taken at 40× magnification 72 h after transfection of the indicated saRNAs. (D) NCCIT cells were transfected with dsNanog-752 for 3 days at concentrations ranging from 10 to 200 nM. Expression of NANOG was assessed by quantitative RT–PCR and normalized to GAPDH. Results are fold changes relative to mock transfections (means±S.D. for three independent experiments). (E) NCCIT cells were transfected with 50 nM dsNanog-752 for the indicated lengths of time. The expression of NANOG protein was detected by immunoblot analysis at each time point. α-Tubulin served as a loading control. Representative blot of n=2.