Fig. 5.
Effect of a GKA on secretion and metabolic profiles of isolated cultured human control islets. A: insulin release patterns with glucose stimulation using stepwise increases of glucose concentrations in the absence and presence of 3 μM piragliatin (n = 3). B: effect of piragliatin on islet respiration dynamics during the glucose staircase, followed by treatment with 5 μM of the uncoupler of Ox/Phos FCCP and 1 mM Na-azide (n = 3). C and D: effect of piragliatin on glucose usage and oxidation of human islets as a function of glucose concentrations (n = 3–4). E: ATP production/insulin secretion diagram. F: changes in intracellular Ca2+ of human islets due to stepwise increases of glucose (n = 3). Hb A1c levels for the 3 controls were 5.6, 5.7, and 5.7%. See legend to Fig. 1 for more information.