Skip to main content
. Author manuscript; available in PMC: 2013 Apr 15.
Published in final edited form as: J Immunol. 2012 Mar 16;188(8):3667–3677. doi: 10.4049/jimmunol.1102460

FIGURE 4. LPS-stimulated HSCs increase the incidence of FoxP3+CD4+ cells in bulk allogeneic CD4+ T cell cultures.

FIGURE 4

Purified bulk BALB/c CD4+ T cells were cultured for 5 d in flat-bottom 96-well plates alone, or with B6 HSCs preincubated without or with 100 ng/ml LPS for 24h at a HSC:Treg ratio of 1:10 for 5 d. T cells were then recovered for examination by flow cytometry. A. HSCs increase the incidence of CD4+FoxP3+Helios+ Tregs. B. Proliferation of FoxP3+Helios+ cells, as assessed by CFSE dilution assay. Bar graph shows the absolute numbers of Tregs in the cultures (n=3). *p <0.05, **p<0.01.