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. 2012 Apr 17;3:132. doi: 10.3389/fmicb.2012.00132

Figure 4.

Figure 4

The mini-CooS from Thermosinus carboxydivorans contains conserved residues essential for CODH activity. An alignment of Carboxydothermus hydrogenoformans CooS-II, the Cdh-type CODH of Methanothermobacter thermautotrophicus (MTH1708), Thermosinus carboxydivorans mini-CooS, and Thermincola potens hydroxylamine reductase TherJR_2940 was constructed. Dobbek et al. (2001) defined three sites as being essential for CooS activity. Cluster C (purple), B (red), and D (green). All of these residues are present in the C. hydrogenoformans CooS-II, M. thermoautotrophicus Cdh, and T. carboxydivorans minimal CooS. Many of these residues are absent in the hydroxylamine reductase. The sequences were aligned with MAFFT v6.861b (http://mafft.cbrc.jp/alignment/server/index.html).