Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: Cell Microbiol. 2011 Aug 31;13(11):1755–1767. doi: 10.1111/j.1462-5822.2011.01656.x

Figure 5.

Figure 5

Individual deletion of seven etramps in blood stages and passage through mosquito and liver stages reveals no critical function in any stage. (A) Generalized schematic representation of the strategy used to replace P. yoelii etramp ORFs with TgDHFR/TS. (B) Genotyping of etramp KO blood stage parasites before (“Input”) and after (“Output”) passage through mosquito and liver stages. The presence of knockout parasites in the output blood stages indicates that each individual etramp is not essential for completion of the parasite life cycle. Specific oligonucleotide primer amplicons indicated in (A) are used to test for integration of the KO construct from the 5’ end or 3’ end, and also to test for the presence of the endogenous etramp ORF. PY03652- and PY04799- parasites were analyzed as clones; the remaining strains were analyzed as mixed parental populations of gene deletion parasites and WT parasites, and therefore show evidence for both 5’ and 3’ integration, as well as the unmodified ORF, in different parasites.