Figure 3.

The cell imaging and fluorescence intensity reflecting the interactions between soluble synthetic Le^X and Le^Y determinants with DC-SIGN receptor on THP-1 leukemia cells, in the immunofluorescence assay. Control THP-1 cells, nondifferentiated (a) or differentiated (b) with PMA, GM-CSF and IL-4 stained with FITC-conjugated sheep antibodies against mouse Igs (secondary Ab). Nondifferentiated (c) and differentiated (d) THP-1 cells stained with mouse anti-DC-SIGN monoclonal antibodies (mAb) and with FITC-conjugated secondary Ab. Differentiated THP-1 cells preincubated with synthetic Le^X (e) or Le^Y (f) determinants before the staining of the cells with anti- DC-SIGN mAb followed by the treatment with FITC-conjugated secondary Ab.