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. 2012 Mar 20;2012:417865. doi: 10.1155/2012/417865

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Copyright © 2012 Laughing Bear Torrez et al.

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Differentiation of cell progenitors associated with the central nervous system (CNS) and the neural retina. (a) Experimental overview for in vitro differentiation of CESCs. (b) Constitutive eGFP expression in differentiated aggregates of cjes001 EBs. (c) Q-PCR analysis of OCT4 and SOX2 pluripotency markers in undifferentiated cjes001 (0-day ESCs) and 7-day EBs. (d) Changes in morphology during in vitro differentiation. Arrowheads indicate EB outgrowth observed 1 week after replating. Neurites resembling neural progenitors (NPs) were formed 10–14 days after replating. Scale bars, 50 μm.