Figure 3.

PKA/AKAP1 fosters while PP2A/Bβ2 retards dendrite outgrowth. A–E, Hippocampal neurons virally transduced at 0 or 7 DIV were fixed and labeled for MAP2B at 7 (A, E) or 17 DIV (A–D); representative images are shown in A. Dendrite complexity was quantified by Sholl analysis as the number of dendritic intersections with 25 μm-spaced concentric circles centered on the cell body (B, E) and by counting primary dendrites (C) and dendritic branch points per neuron (D). Bar graphs show means ± SE of 3–5 experiments with 36+ neurons/condition; *p < 0.05, **p < 0.01, ***p < 0.001. Ctrl, Control.