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Calcium inhibits dendritogenesis. A–D, Hippocampal neurons were cultured in the presence of elevated calcium (2.3 mm), the L-type Ca2+ channel agonist Bay K8466 (50 nm), or the cell-permeant calcium chelator BAPTA-AM (10 μm) and fixed and stained for MAP2B at 15 or 19 DIV as indicated. Representative epifluorescence images are shown in A and C, while results from Sholl analyses are plotted in B and D as means ± SE of three experiments with 36+ neurons/condition; ***p < 0.001.
