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. 2012 Apr 9;209(4):653–660. doi: 10.1084/jem.20111457

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© 2012 Segura et al.

This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).

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Figure 3. — Phenotype of LN DC subsets. (A) Axillary LN cells were enriched for DCs and stained for HLA-DR, CD11c, CD1a, CD14, EpCAM, BDCA1, Clec9A, CD206, CD83, CD86, BDCA3, CD172a, CD209, CCR7, or control isotype. Representative results of 4–12 independent experiments are shown. (B) Purified DC populations were coated on microscopy slides, fixed, permeabilized, and stained for langerin (green). The nucleus is stained with DAPI (blue). Representative images are shown of three to four independent experiments. Bars, 10 µm.