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. 2012 Apr 9;209(4):653–660. doi: 10.1084/jem.20111457

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© 2012 Segura et al.

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Figure 6. — Cell cycle status of blood and lymphoid organ DCs. Blood PBMCs were enriched for DCs and stained for HLA-DR, CD11c, CD16, CD14, BDCA1, and BDCA3. Axillary LN or tonsil cells were enriched for DCs and stained for HLA-DR, CD11c, CD14, BDCA1, and BDCA3. Cells were fixed, permeabilized, and stained for Ki67 and DAPI. BDCA1⁺ DCs were gated as HLA-DR⁺CD11c⁺CD16⁻CD14⁻BDCA1⁺, Clec9A⁺ DCs were gated as HLA-DR⁺CD11c⁺CD16⁻CD14⁻BDCA3⁺, and pDCs were gated as HLA-DR⁺CD11c⁻. (A) Representative results for BDCA1⁺ DCs and pDCs are shown. (B) The percentage of Ki67⁺DAPI^high cells is shown in six independent experiments for blood DCs and nine independent experiments for lymphoid organ DCs (three LNs and six tonsils). Horizontal bars indicate the mean. ***, P < 0.0001.