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. 2012 Apr 9;209(4):855–869. doi: 10.1084/jem.20111721

Figure 5.

Figure 5.

Mitochondrial autophagy is decreased in CSB-deficient cells. (A and B) Western blots of CS1AN cells expressing WT CSB or an empty vector (A) or HSMM subjected to lentiviral knockdown of CSB treated with vehicle, 5 µM rotenone (autophagy inducer), or 500 ng/ml ethidium bromide for 24 h (B; n = 3, data are represented as mean ± SEM). (C) Representative image of CS1AN cells expressing WT CSB or an empty vector transfected with eGFP-tagged LC3 and quantification of the foci per cell (n = 3; data are represented as mean ± SEM). (D) Representative image of CS1AN cells expressing WT CSB or an empty vector treated with vehicle or rotenone and stained for LC3B and COX4 (mitochondria; bars, 50 µm) and quantification of the Pearson’s coefficient of these cells (n = 4; data are represented as mean ± SEM). (E) Representative image of CS1AN cells expressing WT CSB or an empty vector treated with vehicle or rotenone and stained for P62 and COX4 (bars, 50 µm) and the quantification of the Pearson’s coefficient of these cells (n = 4; data are represented as mean ± SEM). (F) Representative image of CS1AN cells expressing WT CSB or an empty vector treated with vehicle or bafilomycin A and stained for ubiquitin and COX4 (bars, 50 µm) and the quantification of the Pearson’s coefficient of these cells (n = 4; data are represented as mean ± SEM).