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. 2012 Apr 9;209(4):775–792. doi: 10.1084/jem.20112422

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© 2012 Vilagos et al.

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Figure 10. — Normal V_H gene repertoire in EBF1-induced B-1a cells. Analysis of the rearranged and expressed V_H gene repertoire of B-1a and FO B cells by RNA-sequencing. The abundance of the 76-nt-long sequence reads, which correspond to the RNA transcripts mapping to the variable (V_H), diversity (D_H), joining (J_H), and constant (C_H) gene segments of the Igh locus, are shown for B-1a and FO B cells of WT and Cd79a-Cre R26CA^Ebf1/+ mice. A schematic diagram of the Igh locus based on the C57BL/6 V_H gene assembly (Johnston et al., 2006) indicates members of the distinct V_H gene families in different colors. Arrows denote the positions of the V_H11.2.53 and V_H12.1.78 gene segments.