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. 2012 Apr 9;209(4):793–806. doi: 10.1084/jem.20112005

Figure 2.

Figure 2.

CD89 is required for complement deposition, leukocyte infiltration, proteinuria, and hematuria. (A) Deposits of MBL and C3 detected by immunohistochemistry in frozen kidney sections of 12-wk-old α1KI-CD89Tg compared with α1KI and WT mice. (B) Immunostaining for the detection of CD11b+ infiltrates in frozen kidney sections of mice. (left) Representative sections. Arrows indicate CD11b+ cells. (right) Numbers of positive cells per glomerulus counted in 20 randomly chosen fields for each mouse at 200 magnification. n = 4 mice per group. Bars, 10 µm. (C and D) Protein to creatinine ratio measured in the urines of 12-wk-old α1KI-CD89Tg mice compared with WT and α1KI (C) and 3-, 6-, 12-, and 24-wk-old α1KI and α1KI-CD89Tg mice (D). 7–20 mice per group. (B and D) Error bars indicate SEM. (E and F) Ratio of albumin to creatinine (E) and hematuria (×10,000 red cells/ml; F) as measured in the urines of 12-wk-old WT, α1KI, and α1KI-CD89Tg mice. 7–14 mice per group. (G) Levels of creatinine in the serum of 12-wk-old α1KI and α1KI-CD89Tg mice. 23–25 mice per group. Bars represent the mean. *, P < 0.05; **, P < 0.01; and ***, P < 0.001 (using the nonparametric Mann-Whitney U test).