Figure 5.

Activation of mutant SKW3.TRBV20 cell lines by different bacterial species. 11 mutant α chain (A) and 14 mutant β chain (B) SKW3.TRBV20 cell lines were tested for activation by E. coli, S. epidermidis, K. pneumoniae, and P. aeruginosa. Shaded bars show the fold increase in CD69 surface expression (fold increase in MFI) of mutant SKW3.TRBV20 cells co-incubated with C1R cells infected with bacteria at an MOI of 100 compared with SKW3.TRBV20 cells co-incubated with uninfected C1R cells (open bars). The experiments shown in A and B were also performed at an MOI of 1 and 10, with similar results (not depicted). These experiments were performed three times, with similar results.