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. 2001 May 1;98(10):5746–5751. doi: 10.1073/pnas.101329598

Figure 1.

Figure 1

PFP loading of GzmA or GzmB induces laminB cleavage. (a) GzmA or GzmB (1 μg) were loaded with PFP into K562 cells at 37°C for indicated times. Electrophoresed samples were probed with laminB mAb (101-B7), which recognizes full-length laminB and a 46-kDa cleaved product in cells treated with either GzmA or GzmB, as well as an unknown cross reactive protein migrating at 50 kDa. GzmA laminB cleavage was not blocked by preincubation with CI, maintained throughout the experiment. (b) GzmA loading of K562 cells generates a 25-kDa C-terminal laminB fragment at 90 min, visualized by using polyclonal Ab against the C terminus of laminB. Reprobing for the GzmA-interacting protein PHAPI (15) controlled for loading.