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. 2001 May 1;98(10):5746–5751. doi: 10.1073/pnas.101329598

Figure 4.

Figure 4

LaminA/C are degraded in isolated HeLa nuclei by GzmA. GzmA-treated nuclei were analyzed by immunoblot probed with anti-laminA/C mAb and Ab to ref-1 as a control for loading. (a) LaminsA/C are degraded with similar kinetics as laminB (Fig. 2). Four-fold dilutions of GzmA from 1.5 to 100 nM were added for the indicated times. Cleavage is blocked by GAI and is not induced by inactive S-AGzmA. GzmB does not induce laminA/C cleavage (not shown). (b) Ser protease inhibitors, but not CI, reduce GzmA cleavage of laminA/C. HeLa nuclei were incubated for 1 h with nothing (mock) or with 100 nM GzmA in the presence of no inhibitor, DMSO, or the indicated inhibitors. The percentage of cleavage in the presence of inhibitors compared to uninhibited enzyme is graphed. (c) LaminA/C cleavage is detected 90 min after PFP loading 4-fold dilutions of active GzmA (0.2–150 nM) into K562 cells.