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. 2012 Apr 17;7(4):e35697. doi: 10.1371/journal.pone.0035697

Figure 1. Detection of allergen conjugation on micro-beads using mouse monoclonal and rabbit polyclonal antibodies.

Figure 1

Panel A: Each antibody preparation was tested on the respective allergen-coupled micro-bead. The other two micro-beads were not in the tube. NP: Not performed in this singlepex testing. * Values are expressed as Median Fluorescence Intensity [MFI]. Upper right corner: an example of blank fluorescence reading of the three conjugated micro-beads without serum. Panel B: Antibodies selected on the basis of the findings reported in panel A were tested on the three allergen-coupled micro-beads, all found in the same testing tube. * Values are expressed as Median Fluorescence Intensity [MFI]. Upper right corner: an example of micro-bead fluorescence scatter plot and micro-bead clusters.