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. 2012 Apr 17;7(4):e35282. doi: 10.1371/journal.pone.0035282

Figure 5. ASIP protein expression in different tissues of bulls with normal expression (−) or over-expression of ASIP mRNA (+).

Figure 5

Chemiluminescence detection of ASIP and β-actin by Western blotting of 40 µg protein of the respective tissues. Lanes 1 and 2: M. longissimus, 3 and 4: subcutaneous fat, 5 and 6: intermuscular fat, 7 and 8: heart, 9 and 10: liver, 11 and 12: lung.