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. 2012 Apr 17;7(4):e35394. doi: 10.1371/journal.pone.0035394

Figure 1. Analysis of expression of VjbR under standard culture conditions.

Figure 1

(A) Western blot analysis. B. abortus 2308 wild type strain was grown in rich medium (TSB). At different times, optical density at 600 nm (OD600) was determined, and samples were subjected to 10% SDS-PAGE, transferred to PVDF membranes, and developed with anti-VjbR or anti-RibH1 polyclonal antibodies. The corresponding optical density at 600 nm (OD600) of each sample was 0.5, 0.7, 1.7, 2.6, 2.95, 3.5, and 3.6, respectively. Results from a representative of two independent experiments are shown. (B) Analysis of the vjbR promoter activity. Strains B. abortus PvjbR-lacZ (white circles) or B. abortus ΔvjbR PvjbR-lacZ (black circles) were cultured in TSB. OD600 and β-galactosidase activities were determined along the growth curve at the indicated times. Values are means ± standard deviations of duplicate samples from a representative of two independent experiments. Dotted lines indicate the best linear fit to the data. *, P<0.05.