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. 2012 Apr 17;7(4):e35544. doi: 10.1371/journal.pone.0035544

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Vanheel et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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A quantitative fluorescent western blot was performed to analyze the presence and expression levels of KCNMA1 (Panel A) and DLG4 (Panel B). By means of peak detection, the normalized peak volumes were used for quantification. No significant difference was found in expression levels, but both proteins were detected in the samples of the 2D-DIGE experiment. All animals were included in the WB analysis; control (C), onset (O), top (T) and recovery (R).