Figure 7. Effect of DMSO treatment on UV-induced apoptosis.

(A) DMSO enhances PARP cleavage caused by UV-irradiation. After DMSO and UV treatments, CL1–5 cells were analyzed by Western blotting with anti-PARP and HLJ1 antibodies. GAPDH was used as an internal control. (B) Quantitative results of three independent western blotting analyses are shown. *P < 0.05, #P  =  0.014, compared with DMSO untreated control. (C) DMSO increases UV-induced apoptosis. CL1–5 cells were pretreated with DMSO and exposed to UV-irradiation. After a 48 h recovery period, the percentages of apoptotic cells were quantified by flow cytometry and presented as the means ± S.D. of three experiments. *P < 0.05, #P < 0.01, significantly different from the cells pretreated without DMSO and exposed to UV irradiation.