Figure 6. PPV-PYCS/MVA-PYCS prime/boost improves cytokine secretion by memory CD8⁺ T-cells.

Groups of BALB/c mice (4 per group) were immunized in prime/boost as indicated in Table 1 and 53 days post boost splenocytes were processed for ICS as described under Materials and Methods. Phenotypic differentiation of CD8⁺ T-cells based on memory markers CD127 (V450) and CD62L (FITC). Each quadrant represents different memory population of CD8 cells with its respective percentages. The distribution of antigen specific CD8+ T-cells secreting cytokines in response to CS peptide stimulation, within the different memory population is also shown. The total CD8+ T-cell response is indicated by the black boxes. The boxes on left indicate the responses towards CS peptide stimulation while the ones on right represent its respective RPMI controls.