Table 1. Dosage of different vaccination regimes: A summary of priming and boosting agents used in the study with the corresponding dosages.

Experiment	Priming		Boosting		Number of mice
	Agent	Dose	Agent	Dose
IFN-γ ELISPOT	VV-PYCS	107 PFU	VV-PYCS	107 PFU	5
	PPV1	10 µg	VV-PYCS	107 PFU	5
	PPV	50 µg	VV-PYCS	107 PFU	5
	PPV	10 µg	MVA-PYCS	107 PFU	5
	PPV	50 µg	MVA-PYCS	107 PFU	5
	PPV	100 µg	MVA-PYCS	107 PFU	5
	PPV	10 µg	VV-LUC	107 PFU	5
	–		MVA-PYCS	107 PFU	5
	nrPPV2	50 µg	MVA-PYCS	107 PFU	5
	PPV	10 µg	PPV	10 µg	5
	PPV	100 µg	PPV	100 µg	5
Intra-cellular Cytokine Staining (ICS)	PPV	50 µg	PPV	50 µg	4
	PPV	50 µg	MVA-PYCS	107 PFU	4
	DNA-PYCS	100 µg	MVA-PYCS	107 PFU	4
	nrPPV	50 µg	nrPPV	50 µg	4
	PBS		PBS		4
Challenge Studies	VV-PYCS	107 PFU	VV-PYCS	107 PFU	5
	PPV	10 µg	VV-PYCS	107 PFU	5
	PPV	50 µg	VV-PYCS	107 PFU	5
	PPV	50 µg	MVA-PYCS	107 PFU	5
	FLU-PYCS	107 PFU	MVA-PYCS	107 PFU	5
	PPV	10 µg	PPV	10 µg	5
	–		–		5

All agents were administered s.c except for DNA-PYCS which was injected intradermally.

¹PPV refers to PPV-VLPs containing the CD8 epitope of P.yoelii CS protein.

²nrPPV refers to non-recombinant PPV-VLPs.