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. 2012 Apr 17;7(4):e34933. doi: 10.1371/journal.pone.0034933

Table 3. PCR array results indicating changes in mRNA levels of T cells after tuftsin treatment.

Fold Change in mRNA levels of pro-inflammatory cytokines and receptors (compared to control group)
Gene Symbol Group 1 (NCM) Group 2 (Tuftsin) Group 3 (Tuftsin+NCM)
Interleukin 10 (IL10) 2.54 1.03 23.42
IL10 receptor (IL10Ra) 0.84 0.70 19.42
IL10Rb 1.09 1.19 6.59
IL13 1.06 0.77 12.73
IL4 1.67 0.79 15.12
Transforming growth factor (TGF) beta 1 1.30 1.24 27.61
Chemokine receptor (CCR)3 0.97 0.84 36.83
CCR5 0.93 31.09 86.96
Interferon (IFN) gamma 1.19 0.84 7.61

T cells were treated with MgCM that was isolated from microglia treated with NCM alone, 100 µg/ml of tuftsin alone, or a combination of NCM+100 µg/ml tuftsin for 10 hours. After 3 days, mRNA was isolated from T cells and was reverse-transcribed and used to probe a PCR array kit of inflammatory cytokines and their receptors to determine the subtype of T cells present as a result of treatment, as described in Methods. n = 2.