Table 3. PCR array results indicating changes in mRNA levels of T cells after tuftsin treatment.
Fold Change in mRNA levels of pro-inflammatory cytokines and receptors (compared to control group) | |||
Gene Symbol | Group 1 (NCM) | Group 2 (Tuftsin) | Group 3 (Tuftsin+NCM) |
Interleukin 10 (IL10) | 2.54 | 1.03 | 23.42 |
IL10 receptor (IL10Ra) | 0.84 | 0.70 | 19.42 |
IL10Rb | 1.09 | 1.19 | 6.59 |
IL13 | 1.06 | 0.77 | 12.73 |
IL4 | 1.67 | 0.79 | 15.12 |
Transforming growth factor (TGF) beta 1 | 1.30 | 1.24 | 27.61 |
Chemokine receptor (CCR)3 | 0.97 | 0.84 | 36.83 |
CCR5 | 0.93 | 31.09 | 86.96 |
Interferon (IFN) gamma | 1.19 | 0.84 | 7.61 |
T cells were treated with MgCM that was isolated from microglia treated with NCM alone, 100 µg/ml of tuftsin alone, or a combination of NCM+100 µg/ml tuftsin for 10 hours. After 3 days, mRNA was isolated from T cells and was reverse-transcribed and used to probe a PCR array kit of inflammatory cytokines and their receptors to determine the subtype of T cells present as a result of treatment, as described in Methods. n = 2.