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. Author manuscript; available in PMC: 2012 Apr 17.
Published in final edited form as: Cell Host Microbe. 2010 Feb 18;7(2):164–175. doi: 10.1016/j.chom.2010.01.009

Figure 1. HSP90 Chaperone Components, but Not TAO1, Are Required for AvrB-Induced Susceptibility and PDF1.2 Expression.

Figure 1

(A) GDA inhibits AvrB-3xFLAG transgene-induced susceptibility. (B) TAO1 is not required for AvrB-induced susceptibility. Plants of the indicated genotypes were pre-treated with estradiol, infiltrated with hrcC mutant bacteria in the presence of GDA or buffer (DMSO), and bacterial populations within leaves determined at the indicated times (A and B). (C) GDA abolishes PDF1.2 induction by the AvrB-3xFLAG transgene. (D) TAO1 is not required for PDF1.2 induction by the AvrB-3xFLAG transgene. (E) GDA inhibits PDF1.2 induction by bacterially delivered AvrB. (F) RAR1 is required for PDF1.2 induction by bacterially delivered AvrB. Arabidopsis plants of the indicated genotype were infiltrated with estradiol for 24 hours (C and D) or the indicated bacterial strains (E and F) for 6 and 9 hours (E) or 6 hours (F), and RNA isolated for quantitative RT PCR. For experiments in C and E, GDA or buffer (DMSO) was co-infiltrated into the leaves. The PDF1.2 expression was determined by real-time RT-PCR. Data in are representative of three independent experiments with similar results.