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. Author manuscript; available in PMC: 2012 Apr 17.
Published in final edited form as: Cell Host Microbe. 2010 Feb 18;7(2):164–175. doi: 10.1016/j.chom.2010.01.009

Figure 3. AvrB Directly Interacts with and Induces Phosphorylation of MPK4.

Figure 3

(A) AvrB-3xFLAG induces MPK4 phosphorylation. Specific anti-MPK antibodies were used to immunoprecipitate MPK4 and MPK6 proteins from estradiol-treated rpm1 plants with (+) or without (−) the estradiol-inducible AvrB-3xFLAG transgene, and the level of dual phosphorylation on MPK4 and MPK6 was determined by immunoblot using anti-phospho-ERK1 antibodies. Equal loading of immunoprecipitated proteins was confirmed by immunoblot using anti-MPK4 and anti-MPK6 antibodies. (B) and (C) Bacterially-delivered AvrB induces MPK4 phosphorylation. Arabidopsis rpm1 plants were inoculated with 108 CFU/ml DC3682 carrying an empty vector (pDSK), WT avrB, avrBT125A, avrBR266G or avrBD297A mutant plasmids for 6 hrs, and the phosphorylation state of the immunoprecipitated MPK4 was determined by immunoblot using anti-phospho-ERK1 antibodies.