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. Author manuscript; available in PMC: 2013 May 1.
Published in final edited form as: Dev Dyn. 2012 Mar 29;241(5):879–889. doi: 10.1002/dvdy.23774

Figure 1. mmy mutants have severe defects in primitive hematopoiesis.

Figure 1

(A). Whole-mount in situ hybridization of myeloid markers (l-pln, lysc, and mpo) at 24 hpf. (B). Expression of erythroid markers αe1 and αe2 globins, band3 and gata1 at 21 somite. (C). Stem cell markers cbfb and scl are also down-regulated in mmy mutants. (D). Acridine orange staining showing extensive apoptosis in mmy mutants through the entire embryos. For all probes tested, we typically used 30–40 embryos per hybridization. As such, 25% (8–10embryos) will be mmy.