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(A). Schematic of the PCR assay used to assess nuclear splicing. Blue arrows indicate the locations of the forward and reverse primers. (B). RT-PCR analysis from 24 hpf wild-type and mmy mutant embryos was performed for the indicated genes (Table S5). Zebrafish genomic DNA (gDNA) was included to show the sizes of the un-spliced products. NTC: no template control.
