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. Author manuscript; available in PMC: 2013 Apr 17.
Published in final edited form as: Anal Chem. 2012 Mar 28;84(8):3593–3598. doi: 10.1021/ac203297z

Figure 3.

Figure 3

Adenosine detection and analysis of SAHH activity using the coupled assay. (A) ADO calibration. Using PPDK (100 U L−1) and AgAK (6 U L−1), ADO is rapidly converted to light. This assay displays a broad dynamic range for ADO (1–80 pmol). (B) Detection limits for the luciferase-coupled assay. The procedure allows ultra-sensitive detection of SAHH activity (10−7 unit per well). (C) Application of the luciferase assay to the determination of human SAHH kinetic parameters. The enzyme displays a Km of 22 ± 2 μM and a kcat of 0.075 ± 0.006 s−1.