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Confirmation of active site binding of the FP probe with chymotrypsin, a serine hydrolase. Assay measures the hydrolysis of the p-nitroanilide from S7388, a chromogenic substrate for chymotrypsin. Chymotryptic activity is not detectable upon incubation with the active site probe for 2 min; whereas, activity is preserved after expose to the inactive probe for 2 hrs.
