Table 1.

Dependence of solubility measurements on equilibration time

Protein	Precipitant	Equilibration time	Solubility (mg/mL)
α-Chymotrypsin	15% PEG-8000	10 min	5.8 ± 0.5
		24 h	5.7 ± 0.2
	2 M ammonium sulfate	10 min	31.9 ± 0.8
		24 h	31.7 ± 0.8
Lysozyme	5% PEG-8000	10 min	14.1 ± 0.2
		24 h	14.1 ± 0.1
	1.5 M ammonium sulfate	10 min	18.2 ± 0.7
		24 h	17.7 ± 0.4
Serum albumin	20% PEG-8000	10 min	9.8 ± 0.2
		24 h	10.1 ± 0.2
	2.5 M ammonium sulfate	10 min	8.9 ± 0.3
		24 h	9.0 ± 0.6
RNase Sa	10% PEG-8000	10 min	17.3 ± 0.6
		24 h	17.2 ± 0.2
	1.5 M ammonium sulfate	10 min	8.9 ± 0.3
		24 h	8.9 ± 0.4
Ovalbumin	7.5% PEG-8000	10 min	22.3 ± 2.1
		24 h	22.2 ± 1.6
	2 M ammonium sulfate	10 min	16.8 ± 0.8
		24 h	16.7 ± 0.6
α-Lactalbumin	25% PEG-8000	10 min	7.8 ± 1.2
		24 h	8.0 ± 0.7
	1.5 M ammonium sulfate	10 min	22.2 ± 0.1
		24 h	22.5 ± 1.3
Fibrinogen	2.5% PEG-8000	10 min	4.5 ± 0.2
		24 h	4.3 ± 0.3
	0.7 M ammonium sulfate	10 min	3.3 ± 0.2
		24 h	3.4 ± 0.2

Experiments were performed in pH 7.0 5 mM citrate buffer.