Table 2.
Parameters obtained for PEG-8000 and ammonium sulfate solubility curves
| Protein‡ | PEG-8000†,§ |
Ammonium sulfate†,¶ |
||||
|---|---|---|---|---|---|---|
| Slope (β) | Intercept (log S0) | R2 | Slope (β) | Intercept (log S0∗) | R2 | |
| α-Chymotrypsin | −0.01 | 0.9 | 0.92 | −0.92 | 2.5 | 1.00 |
| Lysozyme | −0.07 | 1.5 | 0.99 | −1.49 | 3.5 | 0.99 |
| Human serum albumin | −0.13 | 3.6 | 0.99 | −2.68 | 7.5 | 0.97 |
| RNase Sa | −0.04 | 1.6 | 0.99 | −1.63 | 3.4 | 1.00 |
| α-Lactalbumin | −0.13 | 4.2 | 1.00 | −0.17 | 1.6 | 0.98 |
| Fibrinogen | −0.42 | 1.7 | 1.00 | −4.13 | 3.5 | 0.98 |
| Ovalbumin | −0.10 | 2.0 | 0.93 | −2.46 | 6.0 | 0.97 |
†
Equation 2 was fit to the data from Figs. 3 and 4, respectively.
‡
Protein concentration is expressed in units of mg/mL.
§
PEG concentration is express in units of % w/v.
¶
Ammonium sulfate concentration is expressed in units of M.