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. 2012 Apr 18;102(8):1856–1865. doi: 10.1016/j.bpj.2012.03.043

Figure 2.

Figure 2

First-derivative V1-EPR spectra of the 14-PCSL spin label in POPC/PSM binary mixtures (0 mol % chol). With decreasing line height, the spectra correspond to 32.5, 40, 50, 60, 66, 70, 80, 90, and 95.5 mol % PSM contents. Spectra are normalized to constant second integral. In the range 40−95.5 mol % PSM, the EPR spectra form an isosbestic set; I indicates isosbestic points. w and s indicate the outer hyperfine lines of the coexisting weakly and more strongly immobilized components, respectively. T = 23°C.