Table 1.

Thermodynamic parameters for GdnHCl unfolding at 25°C and pH 7 for iso-1-cytochrome c variants

Variant	ΔGo′(H2O)∗ (kcal mol−1)	m-value∗ (kcal mol−1 M−1)	Cm∗ (M)
NH5G-1†	1.7 ± 0.4	4.3 ± 1.0	0.40 ± 0.06
NH5G-2	2.2 ± 0.1	4.5 ± 0.1	0.48 ± 0.03
NH5G-3	2.1 ± 0.2	3.5 ± 0.2	0.60 ± 0.01
NH5G-4	1.7 ± 0.1	2.6 ± 0.2	0.67 ± 0.04
NH5G-5	2.1 ± 0.1	3.3 ± 0.1	0.65 ± 0.02

^∗All parameters are the average and standard deviation of three trials.

^†Thermodynamic parameters are taken from Tzul et al. (22), in which this variant was referred to as Gly5.