Fig. 5.

Immunocytochemical detection of the leptin receptor in adipose tissue using electron microscopy and the immunogold approach. Rat epididymal white adipose tissue fixed in glutaraldehyde and processed for embedding in Lowicryl K4M. Ultrathin tissue thin sections were submitted to the protein A-gold immunocytochemical technique to reveal the leptin receptor. Sections were incubated with the anti-leptin receptor antibody followed by the protein A-gold complex (10 nm gold particles). The labeling by the gold particles is present at the level of the rough endoplasmic reticulum (RER), the Golgi apparatus (G) and some secretory vesicles (v). Very few gold particles can be detected at the level of the lipid droplet (L), the nucleus (N) or the extracellular space (ES). M, mitochondria. Scale bar=0.25 µm.