Fig. 6.

Colocalization of leptin and its own receptor in the same adipocytes. Rat epididymal white adipose tissue 5 µm tissue sections was incubated with the rabbit-anti-leptin antibody and the goat-anti-leptin receptor antibody followed by an anti-rabbit IgG tagged with fluorescein isothiocyanate (FITC) and an anti-goat IgG tagged with tetramethylrhodamine isothiocyanate (TRITC) antibodies. The thin cytoplasmic rim surrounding the lipid droplet and the perinuclear regions display positive signals for both proteins; green for leptin and red for the leptin receptor. Colocalization in yellow confirms that both proteins are present in the same cellular areas. Scale bar=50 µm.