Fig. 9.

Electron microscopy of the gastric chief cells. Immunocytochemistry revealing the presence of leptin in the secretory pathway of the chief cells. The thin tissue sections of the rat gastric mucosa fixed in glutaraldehyde and embedded in Epon were incubated with the anti-leptin antibody followed by the protein A-god complex (10 nm gold particles). The labeling by god particles is found at the level of the rough endoplasmic reticulum, the Golgi apparatus (G) and in the secretory granules (g ). Labeling is also found over the extracellular material of the gastric lumen (L). Scale bars=1 µm (left column), 2 µm (right column).