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. 2011 Jul 27;21(7):1165–1175. doi: 10.1089/scd.2011.0346

FIG. 2.

FIG. 2.

Effect of inhibition of each signaling pathway on the activation of mesoderm-lineage genes in hESCs. Effects of (A) TGF-β signaling inhibitor (SB431542, SB), (B) WNT signaling inhibitor [dickkopf-related protein 1 (DKK1), DKK], and (C) BMP signaling inhibitor (Noggin, Ng) on the expression patterns of mesoderm-lineage genes in hESCs. After 3 days of treatment, real-time PCR was used to measure the expression levels of mesoderm-lineage genes (T, MIXL1, and WNT3a) in the chemical-treated hESCs. Untreated hESCs and ABB-treated hESCs were used as the negative control and positive control, respectively. Statistical significance among samples was evaluated by ANOVA (*P<0.05). ANOVA, analysis of variance.