Figure 1.
Calcium Imaging and simultaneous extracellular recording. A, Simultaneous loose patch recording (top) and ΔF/F0 (bottom recorded from a spontaneously firing neuron. B, Loose patch and calcium indicator fluorescence traces at higher temporal resolution. Note the close linkage between action potential firing (downward deflections in loose patch recording) and onset of calcium indicator response. C, Plot of calcium indicator response relative to action potential firing (vertical line) derived from simultaneous whole-cell patch and calcium indictor imaging. Note the tight relationship between the indicator response and cell firing. D, Correlation between peak ΔF/F0 value and the number of action potentials in neuronal activity recorded from multiple neurons. (n = 3; Pearson r = 0.956, p < 0.0001).