Figure 3. IL-1RI signalling is important for eliciting cytokine and chemokine production in response to CNS S. aureus challenge.
IL-1RI KO and WT mice (n = 8–10 per group) received intracerebral injections of S. aureus (7×103 cfu) and were killed at either 12 or 18 h post-infection. Supernatants from infected hemispheres were evaluated for IL-6 (A), CCL2 (B), CXCL1 (C) and CXCL2 (D) expression by MILLIPLEX analysis with results normalized to total protein concentrations to correct for difference in tissue sampling size. Results are representative of three independent experiments and significant differences between IL-1RI KO and WT mice are denoted by asterisks (***P<0.001).