Fig. 4.

Wild-type Nox4 overexpression results in increased NADPH-dependent superoxide production and phosphorylation of Akt. A: rat PTCs were infected with a full-length active wild-type Nox4 adenovirus (Ad-Nox4), and after 48 h, the lysates were assayed for Nox4 protein expression (A, top) and NADPH-dependent superoxide production (A, bottom). B: a titration of the wild-type Nox4 adenovirus shows a dose-dependent increase in Nox4 protein production concomitant with increased phosphorylation of Akt at serine 473. Ad-GFP was used as a control. Values are means ± SE; **P < 0.01.