Skip to main content
. 2011 Sep 21;302(1):C122–C130. doi: 10.1152/ajpcell.00141.2011

Fig. 5.

Fig. 5.

Nox4-derived reactive oxygen species are required for IGF-I-induced Nox4 expression of fibronectin in PTCs. A: siRNA against Nox4 effectively abrogates the expression of fibronectin at 48 h post-IGF-I treatment relative to control (Scr) siRNA. B: infection of PTCs with an adenovirus expressing dominant negative Nox4 (Ad-Nox4 ΔFAD/ΔNAD) inhibits IGF-I-induced fibronectin expression. C: overexpression of full-length wild-type Nox4 adenovirus (Ad-Nox4) increases fibronectin expression in a dose-dependent manner. Histograms (bottom) represent the intensity of the fibronectin expression to GAPDH quantified by densitometry. Values are means ± SE (n = 3); increased **P < 0.01, or reduced compared with IGF-I induced, #P < 0.05.