Representative recordings of conductance from
Xenopus oocytes expressing different CFTR orthologs. Recordings begin with addition of forskolin/IBMX (10 μM/1 mM) to stimulate conductance. The sequential application of the three increasing concentrations of each inhibitor is indicated by 1 for the lowest, 2 for the medium, and 3 for the highest concentration. A: forskolin/IBMX (10 μM/1 mM) and CFTRinh-172 (5, 10, and 20 μM). B: forskolin/IBMX and glibenclamide (50, 100, and 200 μM). C: forskolin/IBMX and GlyH-101 (5, 10, and 20 μM).